A sample was prepared. It was diluted multiple times to create a wide range of s

Question

A sample was prepared. It was diluted multiple times to create a wide range of solutions with different concentrations. The absorbance was measured and a calibration curve was prepared. Refer to absorbance the spectra and the calibration curve to answer the following questions. Maximum absorbance lambda: Maximum Transmittance lambda: What the color of this solution? An unknown was run with an absorbance of 0.564. What is the concentration of the unknown? If this unknown was diluted, how will it affect absorbance? Will the concentration of the unknown affect the extinction coefficient? Why or why not?

Explanation / Answer

36. The maximum absorbance is indeed 580nm and the maximum transmitance is just 460nm because in 460nm we have the lowest absorbance.

37. The color of the solution is expected to be blue-green because it is the complementary color of yellow (the one who has a wavelenght around 580nm). In this type of problems you need to look up for the complementary color, not the color who has the specific wavelenght.

38. Use the equation and y = 0.564 to get x (concentration).

y = 2.27x - 0.29

0.564 + 0.29 = 2.27x

x = 0.376

39. Indeed, the lower the concentration, the lower the absorbance.

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