7. (a) In the peptide mass mapping technique, proteins can be identified with a

Question

7. (a) In the peptide mass mapping technique, proteins can be identified with a high level of probability even with have low sequence coverage. Explain (b) Give some experimental reasons for low sequence coverage. 7. (a) In the peptide mass mapping technique, proteins can be identified with a high level of probability even with have low sequence coverage. Explain (b) Give some experimental reasons for low sequence coverage. 7. (a) In the peptide mass mapping technique, proteins can be identified with a high level of probability even with have low sequence coverage. Explain (b) Give some experimental reasons for low sequence coverage.

Explanation / Answer

Ans a) Proteins can be identified with a high level of probability evem with low sequence coverage in peptide mass mapping technique. Now peptide mass mapping is a technique that identifies a protein from mass spectrometry data and primary sequence database. By using high level of probability using an appropriate scoring algorithm the closest match can be identified. it does not depends on the sequence coverage. If the unknown protein is present in the sequence database then the aim is to pull out that protein.

Ans b) Some expeimental reasons for low sequence coverage are

protein tolerance, the enzyme chiosen , mass value

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