You have 100 mu L of bacterial stock solution. You take an empty tube \'A\', add

Question

You have 100 mu L of bacterial stock solution. You take an empty tube 'A', add that 100 mu L of bacterial stock and then add 600 mu L of 0 85% saline to it. You will label the tube A as: A. 10^-4 B. 6^-4 C. 7^4 D. 8^-4 E. None of the above If you have a 6X stock solution and you need a 2X solution (using water as a diluent) in a final volume of 30 mL, you will do the following: A. Take 5 mL Stock + 25 mL water B. Take 10 mL Stock + 20 mL water C. Take 25 mL Stock + 5 mL water D. Take 20 mL Stock + 10 mL water E. None of the above In an experiment, a student counts 28 colonies for a 4^-2 dilution with 0.1 mL of the diluted bacterial sample plated. The CFU/mL is A. 28 times 16 times 100 CFU/mL B. 28 times 16 times 10 CFU/mL C. 28 times 64 times 100 CFU/mL D. 28 times 64 times 10 CFU/mL E. None of the above If you do a 8 fold dilution series and label the tubes as: 8^-1, 8^-2, 8^-3, 8^-4, 8^-5, and 8^-6, the dilution between each two tubes going from from left to right is._ A. 2 fold B. 5 fold C. 8 fold D. 10 fold E. None of the above In an experiment, a student counts 90 colonies for a 3^-2 dilution with 0.1 ml of the diluted bacterial sample plated. Assuming that this result is correct and all the steps in the experiment were done correctly, for 3^-4, the student should obtain colonies A. 30 B. 16 C. 10 D. zero E. None of the above If you want to make a 12 fold dilution of a Stock, you will do the following A. Take 1 mL of Stock + 12 mL of water B. Take 1/2 mL (0.5 mL) of Stock + 5.5 mL of water C. Take 12 mL of Stock + 1 mL of water D. Take 5.5 mL of stock + 1/2 mL (0.5 mL) of water E. None of the above If you do a 10 fold dilution series and label the tubes as: 10-1, 10-2, 10-3, 10-4, 10-5 and 10-6, the dilution between each two tubes going from left to right is: A. 2 fold B. 5 fold C. 8 fold D. 10 fold E. none of the above

Explanation / Answer

Ans 1. C. 7-1

Dilution can be calculated using formula,

stock solution / total volume of solution

here total volume = 100 ul + 600 ul = 700 ul

so dilution = 100 / 700

= 7-1

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