You have 100 mu L of bacterial stock solution. You take an empty tube \'A\', add

Question

You have 100 mu L of bacterial stock solution. You take an empty tube 'A', add that 100 mu L of bacterial stock and then add 600 mu L of 0.85% saline to it. You will label the tube A as: A. 10^-1 B. 6^-1 C. 7^-1 D. 8^-1 E. None of the above If you have a 6X stock solution and you need a 2X solution (using water as a diluent) in a final volume of 30 mL, you will do the following: A. Take 5 mL Stock + 25 mL water B. Take 10 mL Stock + 20 mL water C. Take 25 mL Stock + 5 mL water D. Take 20 mL Stock + 10 mL water E. None of the above In an experiment, a student counts 28 colonies for a 4^-2 dilution with 0.1 mL of the diluted bacterial sample plated. The CFU/mL is A. 28 times 16 times 100 CFU/mL B. 28 times 16 times 10 CFU/mL C. 28 times 64 times 100 CFU/mL D. 28 times 64 times 10 CFU/mL E. None of the above

Explanation / Answer

1. When 100 uL of bacterial stock solution is mixed with 600 uL of 0.85% saline. The label would be,

C. 7^-1

2. When 6X solution is diluted to 2X solution with final volume of 30 ml we would need,

B. Take 10 ml stock and 20 ml water

3. In the experiment, the CFU/ml is,

D.

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