You have a 20X solution, and need 1 liter of a 0.5X solution. What volumes of 20

Question

You have a 20X solution, and need 1 liter of a 0.5X solution. What volumes of 20X solution and water do you need? If you need to make a 1 in 10 dilution of a stock solution, and require 50 ml final volume of the diluted solution, how much of the stock solution, and how much water do you need? If you need to make a 0.9% physiological saline solution (sodium chloride), and you need 500 ml final volume, how much sodium chloride do you need? If you need to add Triton-X100 (a liquid) to a solution to a final concentration of 0.5%, in a final total volume of 500 ml, how much Triton-X100 do you need to add? The recipe for 10X PBS calls for 0.1 M Na_2HPO_4, which is anhydrous, but you only have Na_2HPO_4.7H_2O. The molecular weight of Na_2HPO_4.7H_2O is 268.07. If you need to make 500 ml 10X PBS, how much Na_2HPO_4.7H_2O do you need? For live cell imaging, the PBS solution often contains 0.9 mM calcium chloride (molecular weight = 110.98) and 0.49 mM magnesium chloride hexahydrate (molecular weight = 203.3). For 1 liter of PBS, how much calcium chloride and magnesium chloride would you have to add to the standard PBS recipe? The cryoprotectant we use when we store perfused brain tissue sections in the freezer contains 300 g sucrose (molecular weight of 342.3) in an 800 ml volume. What is the % w/v of the sucrose in this solution? What is the molarity of the sucrose solution (Answer to 2 decimal places)?

Explanation / Answer

1.M1V1=M2 V2

M1 =20X V1 = ?

M2 = 0.5 X   V2 =1lit.

   20 * V1 = 0.5 * 1

   V1 = 0.025L

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