You want to amplify a specific DNA sequence from an influenza virus. Which of th

Question

You want to amplify a specific DNA sequence from an influenza virus. Which of the following is necessary for the amplification of this DNA by PCR but not necessary for amplification by cloning?

A) Presence of the recognition sites for a specific restriction enzyme
B) Knowledge of the sequence of at least some parts of the virus DNA
C) A large starting quantity of the virus from which to extract the DNA
D) mRNA from the virus so that noncoding DNA is not amplified
E) The target DNA has to have sticky ends for primer binding.

Explanation / Answer

e is the right answer, in PCR we always need sticky ends i.e 5 or 3 end which forms the primer which finally goes to PCR to make thousands of copies.

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