You want to amplify a 21,666 bp-long fragement of human DNA using PCR. You desig

Question

You want to amplify a 21,666 bp-long fragement of human DNA using PCR. You design a pair of primers that are 21 and 23 bases in length (respectively), have identical melting temperatures, and hybridize correctly to the target DNA sequence . However, when you run your reaction, it fails to produce any product. This is most likely because:

your primers are not the same length

the region of DNA you are attempting to target is too large

your primers are too short

you targeted human DNA; only mouse DNA can be used for PCR

you used more than one primer in the reaction

your primers are not the same length

the region of DNA you are attempting to target is too large

your primers are too short

you targeted human DNA; only mouse DNA can be used for PCR

you used more than one primer in the reaction

Explanation / Answer

b. The region of DNA you are attempting to target is too large PCR amplification is best achieved with 1000 bp to 5000 bp.

c. you used more than one primer in the reaction

d. your primers are too short

e. you targeted human DNA; only mouse DNA can be used for PCR

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