A physiological saline solution, one that is isotonic with tissue is 0.8% w/v (w

Question

A physiological saline solution, one that is isotonic with tissue is 0.8% w/v (weight/volume) NaCl. How would you prepare 1 liter of physiological saline? 1% w/v is the same as 1 g in 100 ml of total solution Calculate the volume of glycerine, which is also known as glycerol, a nontoxic, sweet tasting liquid, that is needed to make 100 ml of a 15% v/v (volume/volume) solution in water. How would you prepare 175 ml of 0.6% w/v agarose in IX TAE buffer starting with agarose powder, a 20X TAE stock solution, and water? IX and 20X refer to the relative concentrations. The following problem is based on a notorious article in Nature, which is one of the most prestigious journals in science (Davenas. E et at (1988) Nature 333.816-818). and has ramifications for homeopathic medicine. Degranulation is a response that is effected by a specific antibody on a suspension of human leukocytes, which are white blood cells. It is a normal immunologic response to foreign antigens In a typical assay for degranulation. 1.5 ml of the antibody solution is mixed with 30 pi of leukocyte cell suspension and then the cells are examined with a microscope in order to determine if degranulation has taken place. According to this article in Nature, a solution of antibody with a concentration of 2 times 10^-9 M causes the degranulation response. When this solution was diluted by a factor of DF = 10^-100, the degranulation response was still observed. Why do you think that these results evoked incredulity among other scientists?

Explanation / Answer

Answer:

Psyological Seline solution has concentration 0.85 (w/v%)

i.e. in 100 mL of solution thre is 0.85 g of NaCl salt dissolved.

I.e. in 1000 mL (here 100 x 10) of solution we expecr 8.5 g (as 8.5 x 10) of NaCl dissolved.

preparation of 1L i.e. 1000 mL physiological seline soluion : Take a 8.5 g of NaCl in 1 L volumetric flask and add some distilled water to dissolve it completely.

Then dilute the solution to 1000 mL by adding requisite amount of=d distilled water.

Hence the preparation of 0.85 % (w/v) pysiological seline solution.

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