The lac operon in E. coli has 3 regulatory regions (the promoter for the repress

Question

The lac operon in E. coli has 3 regulatory regions (the promoter for the repressor, Pr; the operator, O, and the promoter P) and 4 structural genes (the gene for the repressor, i; the gene for beta-galactosidase, z; the gene for galactoside permease, y; and the gene for a transacetylase, a). In the absence of mutation, the structural genes z, y, and a are all expressed in the presence of lactose and repressed when lactose is not present. The genoptye of the wild-type bacterium is: Pr+i+P+O+z+y+a. For the following mutations, would the structural genes be expressed in the presence of lactose? The answer to the first one is given as an example. (4 pts)

Explanation / Answer

Genotype z y a

Pr+i+P+O+z+y-a+ yes no yes

Pr-i+P+O+z+y-a+ no no no

Pr+i+P-O+z+y-a+ no no no

Pr+i+P+O-z+y-a+ no no no

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