The lac operon containsa promoter (P), operator (O), and genes for the lactose m

Question

The lac operon containsa promoter (P), operator (O), and genes for the lactose metabolismenzymes b-galactosidase(Z) and permease (Y). It is regulated by arepressor (I). The promoter (P) region is the start site oftranscription through the binding of the RNA polymerase.Mutationally altered promoters cannot bind RNA polymerase. Thebinding of the repressor (I) to the operator (O) controls access ofRNA polymerase to the Zand Y genes, and a mutant operator (OC) cannot bind therepressor.

For the following conditions, insert a “Yes” in thetable if the bacteria produces an enzyme, and a “No“where no enzyme is produced, and if it would be inducible bylactose or not. The first one is filled in for you as an example.(8pts)

Genotype

b-galactosidase(Z)

Permease (Y)

No lactose

Lactose

No lactose

Lactose

I+P+O+Z+Y+/ I+P+O+Z+Y+

I+ P+O+ Z- Y+/ I-P+ O+ Z+ Y-

I- P-O+ Z+ Y+/ I-P+ OC Z+ Y-

Genotype

b-galactosidase(Z)

Permease (Y)

No lactose

Lactose

No lactose

Lactose

I+P+O+Z+Y+/ I+P+O+Z+Y+

I+ P+O+ Z- Y+/ I-P+ O+ Z+ Y-

I- P-O+ Z+ Y+/ I-P+ OC Z+ Y-

Explanation / Answer

I+ P+ O+ Z-Y+/ I- P+ O+Z+ Y-

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