Safari File Edit View History Bookmarks Window Help 50 56% C40 Thu 11:08 PM a E

Question

Safari File Edit View History Bookmarks Window Help 50 56% C40 Thu 11:08 PM a E Session masteringgenetics.com Principles and Methods of... Mastering Genetics: Genomic... Chegg.com Shotgun sequencing Wik Signed in as Sara Khan Help I Close Molecular Genetics Screen Shot M 201 Screen Shot Genomics: Genomic Cloning and Sequenc Practice Problem 107 2015-0...2.46 PM previous l 2 of 5 l next Practice Problem 107 Screen Shot Part A The first step in annotating a gene is identifying the open reading frame. In bacteria, all genes that code for a protein have a start and stop codon with an open reading frame between them. A bioinformatics program can translate all the possible reading frames (series of 3 codons) in an attempt to find the longest open reading frame. How many possible open reading frames can a bioinformatics program translate from one DNA sequence? Enter your answer numerically (for green Shot example: 2) 5.0.20.34 PM Screen Shot 2015-0...6.41 PM Subm My Answers Give Up Part B Lab Writeup This question will be shown after you complete previous question(s) Part C Screen Shot Screen Shot n5-0...1.24 AM 2015-0...7.01 AM

Explanation / Answer

6

A bioinformatics program can translate 6 possible open reading frames from one DNA sequence.

Any sequence of DNA strand can have 3 possible open reading frames in a single direction and another 3 in reverse direction.

In Frame 1 translation of triplet codon starts with the first nucleotide, frame 2 starts with the second nucleotide whereas in frame 3 starts from third nucleotide in the given DNA sequence. These ORFs are represented as Frame +1, +2 and +3 respectively.

Similarly we get another three ORFs by considering the reverse complement of the given sequence.These reverse complement ORFs are represented as Frame -1, -2 and -3 respectively.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.