A muscle protein in mouse is produced through the use of alternative promoters i
ID: 39711 • Letter: A
Question
A muscle protein in mouse is produced through the use of alternative promoters in heart and skeletal muscle. A diagram of the gene region is shown below. The gene contains a total of 6 exons, and there are three restriction sites recognized by the restriction enzyme HindIII in the vicinity of the gene. In the diagram, the location of heart (PH) and skeletal (PS) promoters are indicated. As are two molecular probes. Probe a hybridizes to exon 2, and probe b hybridizes to exon 4. Transcription of the gene in heart and skeletal muscle terminates after exon 6. The protein produced by the gene is recognized in heart and muscle samples by the same antibody.
a) Diagram the expected results of HindIII digestion of DNA followed by the use of probes a and b in Southern blots from heart muscle compared to skeletal muscle.
b) Diagram the expected results of mRNA when using probes a and b in Northern blots from heart muscle compared to skeletal muscle.
A muscle protein in mouse is produced through the use of alternative promoters in heart and skeletal muscle. A diagram of the gene region is shown below. The gene contains a total of 6 exons, and there are three restriction sites recognized by the restriction enzyme HindIII in the vicinity of the gene. In the diagram, the location of heart (PH) and skeletal (PS) promoters are indicated. As are two molecular probes. Probe a hybridizes to exon 2, and probe b hybridizes to exon 4. Transcription of the gene in heart and skeletal muscle terminates after exon 6. The protein produced by the gene is recognized in heart and muscle samples by the same antibody. a) Diagram the expected results of HindIII digestion of DNA followed by the use of probes a and b in Southern blots from heart muscle compared to skeletal muscle. b) Diagram the expected results of mRNA when using probes a and b in Northern blots from heart muscle compared to skeletal muscle. c) Diagram the expected results of probing the protein content of heart muscle compared to skeletal muscle with an antibody by a western blot.Explanation / Answer
c) Diagram the expected results of probing the protein content of heart muscle Compared to skeletal muscle With An antibody by a western blot.
Because, for the recognition of these proteins is performed with antibodies, and the northern and southern blot applies to nucleic acids, while the western blot applies to protein species, so that the proteins are shown to recognize the heart and muscles by the same antibody.
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