Fill in the following table to show your answers. For the type of ends just ente

Question

Fill in the following table to show your answers. For the type of ends just enter

For each of the restriction enzymes listed below: (i) Approximately how many restriction fragments would result from digestion of the human genome (3 X 10^9 bases) with the enzyme? (ii) Estimate the aver-age size of the pieces of the human genome produced by digestion with the enzyme (iii) State whether the fragments of human DNA produced by digestion with the given restriction enzyme would have sticky ends with a 5' overhang, sticky ends with a 3' overhang, or blunt ends. (iv) If the enzyme produces sticky ends, would all the overhangs on all the ends produced on all fragments of the human genome with that enzyme be identical or not? (The recognition sequence on one strand for each enzyme is given in parentheses, with the 5' end written at the left. N means any of the four nucleotides; R is any purine?that is, A or G; and Y is any pyrimidine?that is, C or T. A marks the site of deavage.) a. Sau3A (AGATC) b. BamHI (GAGATCC) c. BstYI (RAGATCY) d. BsII (CCNNNNN^NNGG) e. NaeI (GCC^GGC) Fill in the following table to show your answers. For the type of ends just enter ??5?? for sticky ends with a 5?? overhang, ??3?? for sticky ends with a 3?? overhang, or ??blunt?? for blunt ends. For whether the ends would be identical enter ??yes,?? ??no,?? or ??NA.?? Fragment Enzyme Size Number Type Identical a b c d

Explanation / Answer

Fragment

        Enzyme            Size         Number         Type          Identical

      

            a 256 bp 11718750 Sticky No

      

            b 4096 bp 732421 Sticky No

            c 4096 bp 732421 Sticky No

            d 4194304 bp 715 Sticky No

            e 4096 bp 732421 Blunt Yes

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