The picture of the gel with size and mass standards should give you an idea of w

Question

The picture of the gel with size and mass standards should give you an idea of what we can see on a gel. If we are amplifying a 100 bp segment of DNA, what is the mass of the 100 base pair DNA band that we hope to see on our gel if we run 45 cycles, for more than needed?

Question options:

10 ng

22 ng

33 ng

66 ng

100 ng

220 ng

330 ng

10 ng

22 ng

33 ng

66 ng

100 ng

220 ng

330 ng

The picture of the gel with size and mass standards should give you an idea of what we can see on a gel. If we are amplifying a 100 bp segment of DNA, what is the mass of the 100 base pair DNA band that we hope to see on our gel if we run 45 cycles, for more than needed? Question options: 10 ng 22 ng 33 ng 66 ng 100 ng 220 ng 330 ng

Explanation / Answer

Based on the given data, here we have initially taken 100 base pair DNA sequence. Hence, the formula for calculation of molar mass of dsDNA fragment is =

(Number of base pairs)× (650daltons per base pair)

= (100) × (650)

= 65000 Dalton’s or g/mol

Thus, a 100 bp DNA has 65000 g/mol    

Then this DNA is amplified for 45 cycles, the formula for calculation is:

= 245 = 3.5184372×1013 molecules of DNA.

Thus, the weight of end product is:

3.5184372×1013 × 65000

= 2.2869842×1017 Daltons.

Upon conversion of Daltons to nomograms we get,

3.862 185 922 500 9×10-12 Nanogram [ng]

Thus, the correct option is (A) 10 ng.

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