Molecular Biology Signaling Help (pt2)!!!!!!! 4. Ste5 binds to the beta and gamm

Question

Molecular Biology Signaling Help (pt2)!!!!!!!

4. Ste5 binds to the beta and gamma subunits of the active G-protein. a. Based on what you know, is this early or late in the signaling pathway? b. If you cross LlSte5 with Ste11, generating an delta Ste5/Ste11 double mutant, the mutant shows the same phenotype as the zlSte5 single mutant. What does this indicate about the signaling sequence? Is it Ste5 right arrow Ste11 or Ste11 right arrow Ste5? c. If you generate a gain-of-function mutant of Ste5 and cross it with Ste11 you get results that are the same as the Kss1 gain-of-function mutant. Given that Ste11 is a gain-of-function mutant, why do we not see these results ?n the Ste11 single mutant (hint: look at what Ste5 encodes and think about what it might do)? Table 2. Shmooing and Fus1 data from the experiment.

Explanation / Answer

a; Kss1 can phosphorylate Dig1 and Dig2, two functionally redundant inhibitors of Ste12 , this shows that kss1 is operates upstream of Dig 1. kss 1 is a map kinases in yeast , where as fus3 gene act as inhibitor. when kss1 gene phosphorylates the Dig1 it cannot inhibhit the ste12 , which is responsible for mating and filamentation.

b.; Ste12 controls two distinct developmental programs of mating and filamentation. Ste12 activity is regulated by Fus3 and Kss1 MAP kinases through two Ste12 inhibitors, Dig1 and Dig2 . here in this case Dig 1 acting as an inhibitor.

c; if Dig 1 gene is non functional ,means it does not control ste12 gene which is responsible for mating ,so phenotype will be continous mating { shooming} occurs.

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