You are studying a new protein. The molecular weight of this protein as determin

Q: You are studying a new protein. The molecular weight of this protein as determin

Answer: The protein is made up of the thee subunits and they are joined by the sulpher bonds, when they are subjected with the 2-mercaptoethaol, there bonds get broken due to action of mercaptoethanol on the supher bonds. so these are separated on the SDS-PAGE into 3 different subunits.

ID: 35696 • Letter: Y

Question

You are studying a new protein. The molecular weight of this protein as determined by gel filtration (size exclusion chromatography) is 350kD. When the protein is subjected to SDS-PAGE, there are 3 bands with molecular masses of 200, 125, and 25. When the protein is subjected to SDS-PAGE in the presence of 2-mercaptoethanol (2-ME), there are 3 bands with molecular masses of 100, 125, and 25.

1. Describe the quaternary structure of the full protein, the number of subunits in the protein, the subunit molecular weight, and any interactions/forces that exist between the subunits.

Explanation / Answer

Answer:

The protein is made up of the thee subunits and they are joined by the sulpher bonds, when they are subjected with the 2-mercaptoethaol, there bonds get broken due to action of mercaptoethanol on the supher bonds. so these are separated on the SDS-PAGE into 3 different subunits.

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