Question 1. if you have just been using the low-power objective, repeat the step

Question

Question 1. if you have just been using the low-power objective, repeat the steps given in direction 7 using the high-power objective lens. What new details become clear?

Question 2. immersion lens to cloud the 40x lens. turn the revolving nosepiece in the other direction to the low-power lens, and recenter and refocus the object. Then move the immersion lens back into position, again avoiding the 40x lens. Sketch the letter e in the summary chart. What new details become clear?

Question 3. is the field again decreased in size?

Question 4. As best you can, estimate the working distance, and record it in the summary chart. Is the working distance less or greater than it was when the high-poer lens was focused?

Explanation / Answer

1.Use only fine focusing control when focusing the higher power objectives on a slide.(20X,40X,100X)

2.The real image is magnified by ocular lens to produce the virtual image seen by eye.Multiple lenses are mounted on nosepiece and light pases through the slide and then into objective lenses.

Record the working distance in the summary chart and then move the immersion lens back into the position.

3.For a fixed object distance and aperture size, increasing the focal length will decrease depth of field.

4.The working distance is greater because if you determine diameter of the field of view you can estimate size of an object.

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