Question 1. Using NCBI (http://www.ncbi.nlm.nih.gov/), answer the following: a)

Question

Question 1.

Using NCBI (http://www.ncbi.nlm.nih.gov/), answer the following:

a)

What is the mRNA sequence for human CRX? Provide your answer in "FASTA" format.

b)

Looking at the "GenBank" format view for the corresponding DNA sequence for human CRX, how long is the sequence?

c)

When was the last time the DNA sequence record for human CRX was updated?

d)

How many exons make-up the coding sequence (CDS) for the Human CRX gene?

e)

What is the protein sequence for human CRX? Provide your answer in "FASTA" format.

Question 2.

Using the NCBI dbEST database (http://www.ncbi.nlm.nih.gov/nucest/), answer the following:

a)

How many EST records are there for CRX?

b)

What species have the ESTs been sequenced from? Recall from the Lecture Slides, if no Tree is available, and you cannot infer from the Title alone, you must open and read each record to determine the “Organism”.

c)

What is the corresponding UniGene Cluster for the EST having Accession = BB371684.1?

d)

Does the EST having Accession = AV361116.1 belong to the same or different Unigene Cluster then BB371684.1?

Question 3.

a)

Given the following GenPept report, provide the "FASTA" format version.

LOCUS NP_001161 342 aa linear PRI 14-AUG-2011

DEFINITION aquaporin-7 [Homo sapiens].

ACCESSION NP_001161

VERSION NP_001161.1 GI:4502187

DBSOURCE REFSEQ: accession HYPERLINK "http://www.ncbi.nlm.nih.gov/nuccore/4502186" NM_001170.1

KEYWORDS .

SOURCE Homo sapiens (human)

ORGANISM HYPERLINK "http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606" Homo sapiens

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;

Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;

Catarrhini; Hominidae; Homo.

Explanation / Answer

1. a. mRNA sequence for human CRX? in FASTA format:

1. b. 4484 bp

1. c. 06 April 2018

1. d. Four exons: 1-171, 172-306, 307-458, 459-4469

1. e. Protein sequence for human CRX in FASTA format:

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