Question 1. Using NCBI (http://www.ncbi.nlm.nih.gov/) answer the following: What

Question

Question 1.

Using NCBI (http://www.ncbi.nlm.nih.gov/) answer the following:

What is the mRNA sequence for human NRL? Provide your answer in "FASTA" format.

Answer = ?

b) Looking at the "GenBank" format view for the corresponding DNA sequence for human NRL, how long is the sequence?

Answer = ?

When was the last time the DNA sequence record for Human NRL was updated?

Answer = ?

d) How many exons make-up the coding sequence (CDS) for the NRL gene?

Answer = ?

e) What is the protein sequence for human NRL? Provide your answer in "FASTA" format.

Answer = ?

Question 2.

Using the NCBI EST database (http://www.ncbi.nlm.nih.gov/nucest/), answer the following:

a) How many EST records are there for NRL?

Answer = ?

b) How many species do these records represent? Look the records over carefully before answering. (Hint, you can also refer to the “Organism Tree” mentioned in the Lecture Slides).

Answer = ?

c) Which species are they?

Answer = ?

d) What is the corresponding UniGene Cluster for EST Accession = BI399001.1?

Answer = ?

Question 3.

a) Given the following GenPept report, provide the "FASTA" format version.

LOCUS CAG46659 153 aa linear PRI 16-OCT-2008

DEFINITION IGF1 [Homo sapiens].

ACCESSION CAG46659

VERSION CAG46659.1 GI:49456677

DBSOURCE embl accession CR541861.1

Explanation / Answer

Homo sapiens neural retina leucine zipper (NRL), transcript variant 1, mRNA

NCBI Reference Sequence: NM_006177.4

GenBank Graphics

b)

Homo sapiens neural retina leucine zipper (NRL), RefSeqGene on chromosome 14

NCBI Reference Sequence: NG_011697.2

Go to:

For getting the sequence here i provided the link below:

https://www.ncbi.nlm.nih.gov/nuccore/NG_011697.2?report=fasta&from=5001&to=41329

d) This transcript has 3 exons, to make-up the coding sequence (CDS) for the NRL gene

e)

neural retina-specific leucine zipper protein isoform 1 [Homo sapiens]

NCBI Reference Sequence: NP_001341697.1

>NP_001341697.1 neural retina-specific leucine zipper protein isoform 1 [Homo sapiens]

Question 2:

a) How many EST records are there for NRL?

Ans:55 EST are there in NRL Gene. (https://www.ncbi.nlm.nih.gov/nucest/?term=NRL)

3) GenBank is the most widely used example of this data base. GenBank Gene Products Data Bank (GenPept), produced by the National Center of Biotechnology Information(NCBI)

a) Homo sapiens insulin-like growth factor 1 (IGF1), RefSeqGene on chromosome 12

NCBI Reference Sequence: NG_011713.1

DEFINITION IGF1:

The insulin-like growth factor-1 (IGF-1) is a pleiotropic growth factor. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. Defects in this gene are a cause of insulin-like growth factor I deficiency.

Related Questions

Navigate

• Browse (All)
• Browse Q
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.