4. You’ve run an interrupted mating conjugation experiment with Hfr cells that a

Question

4. You’ve run an interrupted mating conjugation experiment with Hfr cells that are hmm+ omg+ poo+ and
streptomycin resistant (strR). Your recipient cell is F hmm omg poo and streptomycin susceptible (strS).
Below are the results from that interrupted mating experiment

3. In a test tube you combine competent E. coli cells with your genetically engineered plasmid that contains the gene for antibiotic resistance. You treat the solution and plate your producton antibiotic containing media overnight. The next day you see growth on your plate. Was there horizontal gene transfer? How do you know? If so, what kind of gene transfer? 4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm' omg poo and streptomycin resistant (str Your recipient cel is F- hmm omg poo and streptomycin susceptible (strs) Below are the results from that interrupted mating experiment. 120 E 100 i 80 60 40 20 G th 10 20 30 40 50 time (minutes) strR omgt a) If attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 10 you minutes on a complete media plate that contained streptomycin, would you expect to see growth? b) If you attempted to grow your exconjugants at 20 minutes on minimal media, what other supplement would you have to add to that minimal media in order to see growth? c) Based on these data, draw a chromosomal map of your Hfr donor cell. Make sure you include in your drawing where the origin was located and clearly indicate somehow the direction of gene transfer.

Explanation / Answer

3. A type of Horizantal gene transfer occured, and gave colonies in plate that contain antibiotic. That means the antibiotic resistance gene containing plasmid entered into competent E. Coli cell by Transformation proces and gave Antibiotic resistance. Tranfer of Naked DNA into bacteria with out any mediator like Conjugation tube & virus is called Transformation.

4. a. Yes, 40% of the cells show resistance to streptomycin after 10 min.

b. We need to add poo component to minimal media, because, poo gene not transformed at that time intervel. So at 20 min time, the bacteria are auxotrophs for poo.

C. Origin: Strr- hmm+-omg+-poo+. If any gene transferd in less time, it is first transformed gene. I.e Strr

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.