Looking for detailed explanations for Biochem questions: 20. The following enzym

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Looking for detailed explanations for Biochem questions:

20. The following enzyme type is likely to be a target of regulation in the TCA A. Dehydrogenase B. Isomerase C. Kinase D. Phosphatase E. Answers A and C. 21. In which reaction(s) is GTP (or ATP) directly synthesized in the citric acid cycle? A. conversion of succinyl CoA to succinate B. decarboxylation of -ketoglutarate C. conversion of isocitrate to -ketoglutarate D. conversion of acetyl CoA to citrate E. A and D 22. A new compound isolated from mitochondria is claimed to represent a previously unrecognized carrier in the electron transport chain (ETC). It is given the name coenzyme Z. Which line of evidence do you feel is the most conclusive in assigning this compound a position in the ETC? A. Alternate oxidation and reduction of the mitochondrion-bound coenzyme Z can be readily demonstrated B. Removal of coenzyme Z from the mitochondria results in a decreased rate of oxygen consumption. C. The rate of oxidation and reduction of mitochondrion-bound coenzyme is of the same order of magnitude as the overall rate of electron transfer in mitochondria as measured by oxygen consumption. the electron transport chain. the ETC D. The reduction potential of Z is between that of two compounds known to participate in E. All of the above reasonably support the hypothesis that coenzyme Z is a component of

Explanation / Answer

(20)A.dehydrogenase

The citric acid cycle is regulated primarily by dehydrogenases, the enzymes isocitrate dehydrogenase and ?-ketoglutarate dehydrogenase.

(21)B.Decarboxylation of alpha-ketoglutaric acid

The enzyme succinyl-CoA synthetase catalyzes the fifth reaction of the citric acid cycle. In this step, a molecule of guanosine triphosphate (GTP) is synthesized.

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