My question is the one from the comments. Wouldn\'t the ADP and Pi be on the wro
ID: 3164616 • Letter: M
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My question is the one from the comments. Wouldn't the ADP and Pi be on the wrong side of the inner membrane?
6. Mitoplasts are mitochondria that lack the outer membrane but are still capable of oxidative phosphorylation. Suppose that you were to soak mitoplasts in a pH 7 buffer for several hours. Then, you rapidly isolated the mitoplasts and mixed them in a pH 4 buffer containing ADP and Pt. Would ATP synthesis take place? Explain. (3 pts) Show transcribed image text Expert Answer o Anonymous answered this 143 answers Was this answer helpful? o Yes, Mitoplast are the mitochondria that are capable of the oxidative phosphorylation instead of absence of outer mitochondrial membrane.Rapidly soaking the mitoplast in a pH(4) buffer from p7)having ADP inorganic phosphorus.ATP synthesis takes places because inside the Mitoplast there is basic (pH-7) medium than that of outside acidic (pH-4).Therefore a pH gradient is established which is necessary for ATP synthesis Hide comments Comments You commented That makes sense, but the ADP and Pi are normally in the matrix are. How does the ADP and Pi get into the matrix, which is necessary for ATP production?Explanation / Answer
Yes, here in comment ADP and Pi are mentioned in the matrix which is wrong side. ADP and Pi are present in the cytosol which enters to the mitochondria through an enzyme ADP/ATP translocases during synthesis of ATP by the enzyme ATP synthase. Enzyme ADP/ ATP translocases transport ADP and Pi into the mitochondria and ATP outside the mitochondria. Enzyme synthase has two well defined protein entities that are F0 attached to the inne mitochondrial membrane and F1 situated in the miyochondrial matrix. Enzyme ATP synthase utilizes energy from the proton gradient to convert ADP into ATP.
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