The amount of protein in a sample can be quantified by several kinds of spectrop

Question

The amount of protein in a sample can be quantified by several kinds of spectrophotometric methods. One methods utilize a chromophore-containing reagent and measure light absorbed by protein-reagent complexes. Match the descriptions with the appropriate method Measures protein absorbance directly Measures absorbance of protein-reagent complexes Applies to both methods Detection wavelength of 280 nm. the maximum absorbance for aromatic side chains. Sample concentration is determined by comparison to a standard curve generated with a pure protein. Nucleic adds absorb light at detection wavelength so correction factor must be applied. Absorbance intensity is independent of proem amino add composition. Absorbance is proportions to solute concentration for a fixed path length. Detection wavelength set at maximum absorbance for bound dye. Absorbance intensity is highly dependent on presence of Tarp and Tyr residues.

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Measures protein absorbance directly Measures absorbance of protein reagent complexes Applies to both methods Detection wavelength of 280 nm, the maximum absorbance of aromatic side chains. Detection wavelength set at maximum absorbance for bound die. Sample concentration is determined by comparison to a standard curve generated with a pure protein nucleic acids absorb light at detection wavelength so correction factor must be applied Absorbance intensity is independent of protein amino acid composition. absorbance is proportional to solute concentration for fixed path length Absorbance intensity is highly dependent on presence of Trp and Tyr residues.

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