A student digests plasmid X with restriction enzymes 1 and 2. The student then d

Question

A student digests plasmid X with restriction enzymes 1 and 2. The student then digests plasmid Y with the same two restriction enzymes. The student knows that each plasmid only contains a single recognition site for each restriction enzyme. How many different recombinant plasmids will a ligation reaction consisting of the above generated restriction fragments create? Only consider those recombinant plasmids that are the product of two or fewer restriction fragments in your answer. Your must input your answer as a number. The system does not recognize words. (1 mark) Clarification #1: Restriction enzymes 1 and 2 produce sticky ends that are not complementary to each other (this is analogous to the cloning protocol used in Project 8). Clarification #2: A recombinant plasmid is one that is not the same as either of the originals.

Explanation / Answer

2 one recombinant plasmid for x one recombinant plasmid for y=

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