AGA AGC GGA CUA AUA CUC GCA CGA CCA UCA ACA Guc UAA UAC GUG UAG GCG CGG GACAAC U

Question

AGA AGC GGA CUA AUA CUC GCA CGA CCA UCA ACA Guc UAA UAC GUG UAG GCG CGG GACAAC UGC GAA CAA GGG CAC AUCCUG AAA ARD NCE G HI L K M F P S T W Y V stop 1. You have an 18 nucleotide-long piece of double stranded DNA that contains part of an exon from a gene. This linear piece of DNA can be cleaved into two fragments by the Sall restriction nuclease, whose recognition sequence is shown. This the CMGRHW peptide i) Write a possible nucleotide sequence for this double stranded DNA that satisfies this description. Be sure to indicate the polarity of the strands. Circle the transcribed strand. (8 points) piece of DNA encodes Sall

Explanation / Answer

The sequence could be

tgc atg ggt cga cat tgg which codes for CMGRHW peptide and have SalI site in it(Bold)

The sequence will be

5 ' tgc atg ggt cga cat tgg 3'

3' cca atg tcg acc cat gca 5'

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