AGA AGG GCA CGA UUA UUG CUA AUA CUC AGC AGU CCA UCA ACA CCC UCC ACC GGA GGC GCG

Question

AGA AGG GCA CGA UUA UUG CUA AUA CUC AGC AGU CCA UCA ACA CCC UCC ACC GGA GGC GCG CGG GAC AAC UGC GAA CAA GGG CAC AUC CUG AAA GUG UGA ARDNCEQGH?, L K M F P S T W Y V stop 3. You have the following double stranded DNA sequence 5 '-...CCTCTACGATGTTTGGCATGAG...-3 3'-...GGAGATGCTACAAACCGTACTC...-5 This DNA sequence comes from part of a gene on a chromosome of a eukaryotic organism i) If the top strand of the sequence is the template strand. Write the sequence of the mRNA that is transcribed from this region. Label the 5' and 3' end of this molecule. (5 points) iii) This part of the mRNA is from the 3' end of a gene whose mRNA is 3,000 nucleotides long and contains the signal for bringing release factor to the ribosome during translation. Given this information, what peptide do you predict is encoded by this mRNA fragment? Show your work for full credit. (6 points) iv) If a tRNA has an anticodon that is 5'-CGG-3', write the codon below. Label the 5' and 3' ends for full credit. (3 points) v) What amino acid is charged to the tRNA described in part iv? Write out the full name for full credit (2 points)

Explanation / Answer

5’ CCT CTA CGA TGT TTG GCA TGA G 3’ Template strand

3’ GGA GAT GCT ACA AAC CGT ACT C 5’ Sense strand

3’ GCC 5’ mRNA Codon

Alanine amino acid will be charged to the tRNA that is Ala-tRNAAla OR Arg-tRNAArg

because the anticodon at the third base pair is G, therefore, it may bind with the codon at the third base pair C or U i.e. wobble phenomenon

Therefore the tRNA the two amino acid (Alanine or Arginine) may amino acyl tRNA i.e.

Ala-tRNAAla OR Arg-tRNAArg

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