1) Which of the following statements about restriction endonucleases is correct?
ID: 281003 • Letter: 1
Question
1) Which of the following statements about restriction endonucleases is correct? a) They are enzymes that are present in prokaryote:s b) They catalyze the hydrolysis of phosphodiester linkages at specific points within the nucleotide base sequence of the DNA strands c) Both a and b are correct d) They are not present in prokaryotes e) They have no tertiary structure 2) Restriction fragments of DNA are separated from one another by which process? a) Filtering b) Gel electrophoresis c) chromatography d) microscopy 3) What two enzymes are needed to produce recombinant DNA? a) Endonuclease, dehydrogenase (b) Polymerase, kinase c) Helicase, gyrase (d) Endonuclease, ligase 4) What is a plasmid? a) A vehicle for the insertion of recombinant DNA into bacteria b) Recognition sites on recombinant DNA strands c) Surfaces for protein synthesis in eukaryotic recombinants d) Surfaces for respiratory processes in bacteria 5) Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme that creates fragments with sticky ends? a) AAGG b) AGTC c) GGCC d) ACCA TTCC TCAG CCGG TGGTExplanation / Answer
1) both a and b
they are the enzyme present in prokaryotes they helps in the cleavage of DNA through a process restriction.
2) Gel electrophoresis.
restriction fragments of DNA are sperated to one another by Gel electrophoresis.electrophoresis is a seperation method in which charged particles or molecules migrate through the solution under the influence of electric field.
Dna fragments are negatievly charge molecules because of the phosphate ion, therefore it migrate towards the positive electrode.
3) endonuclease and ligase
the enzymes that help to produce recombinant DNA are restriction endonuclease which cleaves and build new dna fragments and DNA ligase which attaches the dna fragments.
4)a vehicle for the insertion of recombinant DNA into bacteria.
plasmid is a small duble stranded Dna molecule circular in morphology which acts as a vector for the transport of recombinant DNA in bacteria.
5)AAGG
TTCC
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