1) Which of the following is typically not involved in SDS-PAGE? Select one: a.
ID: 270038 • Letter: 1
Question
1) Which of the following is typically not involved in SDS-PAGE? Select one: a. Uniform charge of particles. b. The use of agarose as the gel medium. c. The organization of polypeptides based on size of fragments. d. The disruption of noncovalent linkages between polypeptides. e. The use of polyacrylamide as the gel medium.
2)Agarose gel electrophoresis differs from SDS-PAGE in all of the following ways except:
a. Its resolution is lower.
b. Visualization is generally accomplished using ethidium bromide or one of the less toxic alternative stains such as GelRed.
c. Agarose is extracted from seaweed.
d. Its loading buffer generally gives the sample a uniform negative charge.
e. It is generally run horizontally.
3)
What kind of gel medium is used in SDS-PAGE?
Select one:
a. Agarose
b. TBE
c. Ethidium Bromide
d. FITC
e. Polyacrylamide
Explanation / Answer
1.
The use of agarose as the gel medium is typically not involved in SDS-PAGE.
Hence, the correct answer is (b).
2.
Agarose gel electrophoresis differs from SDS-PAGE in all of the following ways except that its loading buffer generally gives the sample a uniform negative charge.
Hence, the correct answer is (d).
3.
Polyacrylamide kind of gel medium is used in SDS-PAGE.
Hence, the correct answer is (e).
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