2.) Translation (26 points) A) You determine the length of an open reading frame
ID: 278197 • Letter: 2
Question
2.) Translation (26 points) A) You determine the length of an open reading frame by Sanger sequencing. Sanger sequencing resuits often contain errors so you sequence the ORF three times ORF length 1st try: 302nt ORF length 2nd try: 300nt ORF length 3rd try: 301nt Which try likely shows the correct length? Explain (20 words) B) Name 3 GTP binding proteins involved in Translation. Explain their function (20 words per protein) C) Does Anticodon/Codon binding always adhere to perfect A/T-C/G base pairing? Explain (20 words)Explanation / Answer
2) ORF length is usually a multiple of 3 nucleotides ( one start codon, one stop codon and and in between the two, triplet coding region). So, 300nt should be the correct answer.
B) IF proteins i.e initiation factors initiate translation by attaching tRNA to small subunit of ribosome, joining two ribosomal subunits and catalyzing the anticodon-codon reaction..
EF proteins i.e elongation factors help in elongation of polypeptide chain by catalyzing the formation of peptide bond and movement of ribosome along mRNA.
RF proteins i.e releasing factors release the completed polypeptide. RF1 is specific for UAG and UAA termination codons and RF2 is specific for termination codons UAA and UGA.
C) According to WOBBLE hypothesis, if the first base of anticodon is C or A, it will follow the base pairing rule but if it is G, U or I, then they can combine with C,G or A,U or A,C,U codon bases respectively. { for the sake of understanding, bold, italics and bold-underline is used to show the relationship of anticodon and codon}.
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