6.) Post-Translational Modification (14 points) The AKT protein can be found in
ID: 277735 • Letter: 6
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6.) Post-Translational Modification (14 points) The AKT protein can be found in unphosphorylated (AKT) and phosphorylated (pAKT) form. The Western Blot below was generated by treating Liver cells with Insulin for the time indicated on top of the lanes. The cells are then lysed and equal amounts of total protein is run on a gel, blotted and then stained with antibodies against either AKT and pAKT a) Describe the banding patems you see. (20 words) Time after adding Insulin 0 2 1015 30 pAKT AKT b.) Explain the effect of Insulin on AKT and pAKT levels. (20 words) c.) Based only on the effect of Insulin on pAKT levels, can you infer if AKT is activated or deactivated by Insulin? Explain your reasoning. (20 words)Explanation / Answer
a. From the given graph, it is clear that phosphorylated AKT levels increase upon the addition of insulin. This increased pAKT levels are not due to increased AKT levels by translation/transcription as the unphosphorylated AKT levels do not change upon the addition of insulin. So, it appears that insulin mediates the phosphorylation of AKT.
b. a. From the given graph, it is clear that insulin does not change AKT levels by transcription/translation. It only increases the pAKT levels.
c. AKT is activated by phosphorylation. So, insulin activates AKT function.
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