Group Observations: Unknown 1 Unknown 2 Unknown4 Unknown 3 indicated 0, indicato
ID: 275715 • Letter: G
Question
Group Observations: Unknown 1 Unknown 2 Unknown4 Unknown 3 indicated 0, indicatod O relationship indicated 0 relationship indicated O trict Aerobe -needs O& to underg erobic respirohon Not and strict Anerobe ot roughoo no grouh Applications Questions 1 a) Did any of the organisms inoculated into your TSA deeps produce gas?If yes, which ones? strict anoobe b) What sort of metabolic pathways produce such gases, and why? e) Could gas production be associated with virulence? a) ..did any colonies tested turn out to be strict anaerobes? Which? b)...did any colonies turn out to be strict aerobes? Which? c) ...did any colonies turn out to be facultative anaerobes? Which? d) ...did any colonies turn out to be aerotolerant anaerobes? Which? 2. Now that you have seen the TSA deep results...Explanation / Answer
Ans 1) a) Yes, the strict anaerobe is the one which is able to produce gas when being inoculated in the TSA deep. It is because upon inoculation, it leads to the production of the carbon dioxide during its development and growth process.
Ans b) The carbon dioxide is produced as a by-product and this is a part of the whole process of anaerobic respiration. There is substrate level phosphorylation leading to the formation and carbon dioxide is the byproduct.
Ans c) Sometimes, there is a chance the production of excess gas is a sign of virulence. It is the toxins released as gas.
Ans 2) a) The last one is the strict anaerobes because there was no growth observed or seen due to the presence of the oxygen.
b) The first one is strict aerobe as there is good growth observed in presence of the oxygen.
c) The 2nd one is facultative anaerobes as there is some growth observed in presence of the oxygen.
d) The 3rd one is aerotolerant anaerobes where growth is not seen but tolerance is still observed in presence of oxygen.
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