102 PART B: Capsule Staining Procedure your observation of all slides under oil

Question

102 PART B: Capsule Staining Procedure your observation of all slides under oil immersion, record your results in the chart 1. Make drawings of a representative microscopic field of each preparation. 2. Record the comparative size of the capsule, that is, small, moderate, or large. 3. Indicate the color of the capsule and the cell on each preparation. +Refer to the color-plate insert for illustration of this staining procedure L. E. serogens Draw a representative n Capsule size Color of capsule Color of cell REVIEW QUESTIONS 1. Why is heat necessary in spore staining? 2. Explain the function of water in spore staining. Differential Staining for Visualization of Bacterial Cell Structures

Explanation / Answer

1) Heat is needed in spore staining to help the primary stain ( malachite green) penetrate the endospore.

2) Water is used to decolourise the primary stain from the vegetative cell.but the stain is fixed inside the spore.

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