need help on finishing transformation of E. coli with pGLO plasmid on # 1-7 plea
ID: 271706 • Letter: N
Question
need help on finishing transformation of E. coli with pGLO plasmid on # 1-7 please!
Transformation of E. coli with pGLO plasmid 1. What is transformation? Which scientist's experiment demonstrated this (and what was the experiment)? We 2. What does it mean for a cell to be competent? How can cells be made competent? Which method did we 3. The pGLO plasmid we used was recombinant DNA. What are all the relevant components of this plasmid were transforming E. coli cells by giving it what characteristie? use? that was necessary for gaining successful results (transformation of E. coli)? 4. Media with ampicillin was used for what purpose? 5. Media with arabinose was used for what purpose? 6. What temperature was used to "heat-shock" the cells and why did we do this? 7. Why did we grow cells on LB media alone?Explanation / Answer
Answer:
1) What is transformation? Which Scientist's experiment demonstrated this.
2) What does i mean for a cell to be competent? How can cells be made competent? What method did we use?
3) The pGLO plasmid we used was recombinant DNA. What are all the relevant components of this plasmid that was necessary for gaining successful results?
4) Media with ampicillin was used for what purpose?
5) Media with arabinose was used for what purpose?
6) What temperature was used to “heat shock” the cells and why did we do this?
7) Why did we grow cells on LB media alone?
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.