primer 1 is GACTGTACGTACHTA primer 2 IS NOT AATTAAA..... is primer 2? TACAACTACT

Question

primer 1 is GACTGTACGTACHTA

primer 2 IS NOT AATTAAA.....

is primer 2? TACAACTACTTACCA? explain why? if not then what is it?

1.) PCR a.) Using the dsDNA sequence 1 as a template, you want to generate dsDNA sequence 2 using PCR. What are the sequences of the two primers you use? 15nt of each primer have to anneal perfectly to the template. 25 points) Sequence 1 Sequence 2 Priner 1 5 Primer 2 5-TATAGGACGATGTTGATGAATGGTACAATCACAGTACGTACGTACAGTCAGTGAAA-3 5-cr CGTCGTCATGTTGATGAATGGTACAATCACAGTACGTACGTACAGTCTTTAATT-3

Explanation / Answer

primer 1 5' TATAGGACGATGTTG 3'

PRIMER 2 5' ATGTCAGAAATTAA 3'

PRIMER ONE CAN BIND DIRECTALY WHERE AS REVERSE COMPLEMENT WILL BIND AS REVERSE PRIMER.

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