ou are a student in a plant biology lab that is studying the role of a potential
ID: 268284 • Letter: O
Question
ou are a student in a plant biology lab that is studying the role of a potential gene whose protein product helps the plant white lupin from getting damaged to frost in on very cold ngs. You call this gene Anti-freeze-1. It seems to work by being secreted from the leaves of the plant and stops dew for analyze it in greater detail. You grind up some of the lupin leaves, extrac mation. You need to clone the Anti-freeze-1gene to t genomic DNA e plant cells and digest the gDNA separately, with two different restriction enzymes: in separately, with nd digest the vector, aga the same two enzymes. After running the gel, you get the following results: ( 21 Points) 8.0 Lane 1: 1 kb Std in kb Lane 2: Pst I (Lupin) Lane 3: Sma I (Lupin) Lane 4: Pst I (Vector) 5.0 3.0 1.0 0.5Explanation / Answer
A)Sma1. Because digeting with it resulted in a single band of vector as opposed to 2 in case of Pst1 i.e. the vector has 1 site for the former and sites for the latter. For cloning one needs to linearize the vector and hence strictly require a restriction enzyme which cuts it only once.
B) that's because the living genome would have numerous recognition sites for Pst1 along it's genome randomly. Hence the multiple bands
C)that's probably because it is a eukaryotic protein and might require certain post-translation modifications for its activity.
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