what is ABCD mean 140 _Water RNV[Rhodamine B] 70 550 600 650 700 PLGA Shell Wave
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what is ABCD mean
140 _Water RNV[Rhodamine B] 70 550 600 650 700 PLGA Shell Wavelength (nm) 140 40 -Water _RNV[Calcein] C 70 20 630 720 Wavelength (nm) NA R Figure 1. Characterization of the RNV encapsulating calcein, rhod amine B and siRNA. A) TEM image of a hollow core-shell RNV. Scale bar, 50 nm. B) Emission spectrum of RNV loaded with calcein excited at 455 nm. C) Emission spectrum of RNV loaded with rhodamine B excited at 556 nm. D) Gel retardation graph of free siRNA, lysed RNVIS? RNAI, and RNVIsi RNAL Lysed RNV refers to the RNV destroyed by three cycles of freeze-thaw and sonication to release the siRNA inside the water coreExplanation / Answer
A.Fig A shows transmission electron microscopy image of a hollow structured rigid nano vesicle with a bright water core and intact PLGA shell.
Fig B, C are emission spectrum graphs showing fluorescence intensity vs. wavelength. Hydrophilic reagents like calcein, thiamine B, siRNA are encapsulated into the water core of RNV with microfluidic chips. With the help of spectrofluorometer and microplate reader entrapment efficiency and entrapment of calcium or rhodamine B into RNV.
Fig. D shows gel retardation due to entrapment of siRNA into RNV and quantifies the entrapment efficiency. Three groups are compared viz. free siRNA, lysed RNV loaded with siRNA, and untreated RNV loaded with siRNA
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