You have performed yeast two hybrid assay to identify proteins that interact wit

Question

You have performed yeast two hybrid assay to identify proteins that interact with Superman and identified a gene coding for kryptonite that interacts with Superman. What experiments will you perform to demonstrate that the Superman interacts with kryptonite in-vivo? You have performed yeast two hybrid assay to identify proteins that interact with Superman and identified a gene coding for kryptonite that interacts with Superman. What experiments will you perform to demonstrate that the Superman interacts with kryptonite in-vivo?

Explanation / Answer

To show the in interaction invivo, one can perform Immunoprecipitation experiment.

Prepare the plant total protein extract. Then using antibody of superman do Immunoprecipitation. Now with the help of western blotting if Kryptonin band is appearing, that means the proteins are interacting invivo also.

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