You have performed the following trihybrid test cross in maize with the colorles

Question

You have performed the following trihybrid test cross in maize with the colorless (c), waxy (wx) and shrunken (sh) loci. You need to figure out the map order and distance between the loci Cc Wxwx Shsh Colored, Starchy, Plump X CC wxwx shsh (tester) colorless, waxy, shrunken You record the following progeny: Trihybrid Gamete # of Progeny Phenotype of Progen Colored, Starchy, Plum colorless, waxy, shrunken colorless, waxy, Plum Colored, Starchy, shrunken colorless, Starchy, Plum Colored, waxy, shrunken colorless, Starchy, shrunken Colored, waxy, Plum Total C Wx Sh c wx sh c wx Sh 4 2 2708 2538 626 601 116 113 6708 C wx slh

Explanation / Answer

Parent - c wx SH
       - C WX sh

Double cross over (DCO) C WX SH
                         c wx sh

The given order is wrong. Because cross between wx and sh (SCO) in parent gives a DCO

So, the order is c sh wx

The distance between sh and wx = 601 + 626 + 4 + 2 / 6708
           = 0.184 = 18.4

So option E is correct

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