5. Here is thesequence of a gene and the amino acid sequence of the protein that

Question

5. Here is thesequence of a gene and the amino acid sequence of the protein thatit encodes.

Design twooligonucleotide primers to change the isoleucine at amino acidresidue 21 to

valine by the sameprotocol used in lab. Appendix 4 in your lab manual lists thesingle-letter

amino acid code, andthe table of codons. The primers that you design must:

• be at least25 nucleotides long with the change flanked by at least 10nucleotides that

are a perfectmatch

• be at least40% GC

• terminate (atthe 3' end) in G or C.

ATGGCCGAATTACCCATAGCACCAGTTGGGAGAATCATAAAAAATGCCGGTGCCCAGAGAATA

M A E L P I A P V G R I I K N A G A Q R I

AGCGACGACGCAAGGGAAGCACTTGCAAAGATTCTGGAAGAAAAAGGCGAAGAAATAGCAAAA

S D D A R E A L A K I L E E K G E E I A K

GCCGCTAAAAAGTTATAG

A A K K L STOP

What tripletencodes isoleucine at position 21? ________ATA__________

Other primer______________________________________________

Explanation / Answer

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