1. A gene X contains the following piece of sequence close to the transcription
ID: 261514 • Letter: 1
Question
1. A gene X contains the following piece of sequence close to the transcription starting site 5' - CCTCCAAAGAAGAAAAGGAAGGTC 3 A. (5 pts) Write the mRNA sequence, translate into amino acid sequence. B. (5 pts) You are using immunofluorescence staining to visualize this protein in the cell. Predict the subcellular localization of this protein C. (5 pts) Design an experiment to test the hypothesis that this signal sequence is sufficient to direct protein X to the destination D. (5 pts) In a point mutation the underlined "A" is changed to "C Re-write the amino acid sequence, and predict the subcellular localization of the mutated protein.Explanation / Answer
1.
5' - CCT CCA AAG AAG AAA AGG AAG GTC - 3'
3' - GGA GGU UUC UUC UUU UCC UUC CAG - 5'. - mRNA
GGA - glycine
GGU - glycine
UUC - phenylalanine
UUC - phenyalanine
UUU - phenylalanine
UCC - serine
UUC - phenylalanine
CAG - glutamine.
3' - gly - gly - phe - phe - phe - ser - phe - gln - 5''. - protein
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