QUESTION 1 How many colony forming units per mL are there in the following cultu

Question

QUESTION 1 How many colony forming units per mL are there in the following culture? The original bacterial culture was diluted 1:10,000. Then 0.01 mL were spread on each of 3 agar plates. After 16 hours the colonies were counted on the 3 plates and were found to have 20, 25, and 30 colonies respectively. QUESTION 2 How many colony forming units per mL are there in the following culture? The original bacterial culture was diluted 1:1,000. Then 0.05 mL were spread on each of 3 agar plates. After 16 hours the colonies were counted on the 3 plates and were found to have 80, 70, and 90 colonies respectively.

Explanation / Answer

I believe the correct answers to be

A1) 25x106 cells/mL in the original culture

A2) 16x105 cells/mL

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