A gene encodes a protein with the following amino acid sequence: Lys-Met-Pro-Ser

Question

A gene encodes a protein with the following amino acid sequence: Lys-Met-Pro-Ser-Ala-Pro-Thr A nonsense mutation from a two base pair substitution occurs in this gene, resulting in a protein with the amino acid sequence Lys-Met. An intergenic suppressor mutation allows the gene to produce the full length protein. With the original mutation and the intergenic suppressor present, the gene now produces a protein with the following amino acid sequence:

Lys-Met-Ser-Ser-Ala-Pro-Thr

Give the location and nature of the original mutation and of the intergenic suppressor.

Explanation / Answer

WT protein sequence: Lys-Met-Pro-Ser-Ala-Pro-Thr
mRNA sequence: AAA-AUG-CCG-UCG-GCG-CCG-ACG

Mutant protein sequence: Lys-Met
mRNA sequence: AAA-AUG-UAG-UCG-GCG-CCG-ACG
Substitution = CC to UA

Suppressor protein = Lys-Met-Ser-Ser-Ala-Pro-Thr
mRNA sequence: AAA-AUG-UCG-UCG-GCG-CCG-ACG
Substitution = A to C

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