1. You\'ve recently discovered the sequences for two alleles for the mouse gene
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1. You've recently discovered the sequences for two alleles for the mouse gene XCD, which you have creatively named XCD1 and XCD. This gene is believed to be involved in fat metabolism, somehow, but you haven't successfully been able to isolate the protein yet. You've also noticed that your mutant grey coat mice tend to be much leaner and muscular than those with the standard white coat. So for your pilot descriptive experiment with this gene, you'd like to determine if one coat color is more likely to have one allele over another. You're going to explore this hypothesis using a Southern blot. a) Why is a Southern blot the better choice than a Western blot at this point of knowledge about the gene? (1-2 sentences) b) You've already isolated and purified DNA samples from both grey and white mice and have your gel set ups ready to go. What else do you need to do prepare for your Southern blot? c) Your results are presented here for both XCDt and XCD2 alleles. Based on this pilot information, do you think it's likely that different alleles for the XCD gene could contribute to leanness/body wide energy balance? Explain your answer. (2-3 sentences) XCD1 white XcD2 white 8 grey greyExplanation / Answer
1. Western blot is done for separation and analysis of protein. But here the proteins has not yet been isolated. It is difficult to analysis the results of Western blotting by unisolated and unpurified proteins. So, Southern blot is better choice here than the Western blot.
2.
A. The purified DNA must be denatured by gentle treatment of detergent to convert the double stranded DNA to the single strands.
B. The single stranded DNA is fragmented to smaller pieces by the action of restriction endonuclease (RE).
3. Both the XCD1 and XCD2 genes from the white and grey mouse show similar types of band pattern. So, we can say the allele may be the same in their nucleotide sequence. Both the alleles isolated from different mouse are same and. So, the alleles might not have any influence in their body structure.
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