1. You were given a sputum sample from a patient. Design an experiment on how yo
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Question
1. You were given a sputum sample from a patient. Design an experiment on how you would undergo the identification of the possible microbiological agent. You can use any previous laboratories or even outside reliable references. Be sure to include any medias, testing, characteristics that would be useful. But before you are able to identify - what must to obtain from the sputum (hint some type of culture - but what type???)
2. How are infectious diseases different from other diseases?
3. Explain several ways that true pathogens differ from opportunistic pathogens?
4. Compare and contrast systemic vs local infections; primary vs secondary infections
5. What are universal precautions? Why are they critical in your and patient protection?
6. BONUS (PLUS 5 points!!! ) Look into the current literature and news - has there been an incident in the past where universal precautions were not observed - what happened? Discuss the accident, how were precautions not followed, what was the result of the precautions not being followed, and what should have been done differently. What has changed because of the incident. Please cite your article/information and add a copy of the link.
Explanation / Answer
1. The sputum sample could be used for identification of the culture and the organism present in the sample. Sputum samples are usually tested to identify the infections associated with lower respiratory tracts. Sputum could be defined as the phlegm or the thick mucus that would be expelled from the lower portion of the respiratory tract. While collection, it Always has to be taken care that sample collection should be from the lower portion of the tract and not the upper areas of the tract. There would be pathogen detection by susceptibility trysting so that the antibiotic treatment could be guided. This is also done in the form of gram stain, for typical bacterial identification followed by nutrient media placement and incubation. There would be other tests such as fungal culture or afb smear or viral culture.
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