A biology 207 student sub cloned the promoterless, GFP gene from pCam R -GFP int

Question

A biology 207 student sub cloned the promoterless, GFP gene from pCamR-GFP into pUC18-207 using a double digest. They used competent, prototrophic DH5?E. coli cells in their transformation protocol and plated the transformation reaction onto minimal medium that contained additional supplements to help them screen and select for their desired transformants, which are transformed E. coli cells that express the GFP gene. All experimental procedures were followed such as incubation times etc. Note that GFP encodes for a protein that fluoresces green. All you need to answer the questions that follow are the diagrams of the plasmids below and the information throughout this problem.

What percent of the GFP containing pUC18-207 plasmids would express a functional GFPprotein if the biology 207 student successfully inserted the GFP gene into pUC18-207 using a single digest? Assume the GFP gene is promoterless.

a) 66.6%

b) 50%

c) 25%

d) 33%

e) Unknown because a different percent would be observed each time the procedure was completed.

f) <2%

g) 0%

h) 100%

Explanation / Answer

What percent of the GFP containing pUC18-207 plasmids would express a functional GFPprotein if the biology 207 student successfully inserted the GFP gene into pUC18-207 using a single digest? Assume the GFP gene is promoterless.

b) 50%

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.