A biochemist purifies a new protein and wishes to learn something about its stru

Question

A biochemist purifies a new protein and wishes to learn something about its structure. Her results:

The protein has

a. One polypeptide chain with at least 2 disulfide bonds.

b. One polypeptide chain and only 1 disulfide bonds.

c. Two polypeptide chains linked by at least 2 disulfide bonds. d. Three polypeptide chains linked by 2 or more disulfide bonds. e. Three polypeptide chains and no disulfide bonds.

Experiment Number and size of polypeptide(s) by SDS-PAGE None 1;MW 60,000 Chymotrypsin 1;MW 60,000 Performic acid 1;MW 60,000 reduction then carboxy methylation, followed by chymotrypsin 3;MW 30,000, 12,000, 18,000

Explanation / Answer

e)Three polypeptide chains and no disulfide bonds.

because the protein reaction with the components...

and no disulfide bonsd because his size is more big everytime that you change the reactors

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