1.Counting cells is an important part of many immunological assays, as is calcul
ID: 253141 • Letter: 1
Question
1.Counting cells is an important part of many immunological assays, as is calculating dilution factors. a.You have 250 µl of a cell suspension. If you diluted 10 uls of that cell suspension into 90 µl of buffer, what would the dilution factor be? b.You then put 10 µl of that solution into the hemocytometer and you count 47 cells in the appropriate squares. Calculate the cells/ml. c.Calculate how many total cells you had in the original 250 µl of cell suspension. 1.Counting cells is an important part of many immunological assays, as is calculating dilution factors. a.You have 250 µl of a cell suspension. If you diluted 10 uls of that cell suspension into 90 µl of buffer, what would the dilution factor be? b.You then put 10 µl of that solution into the hemocytometer and you count 47 cells in the appropriate squares. Calculate the cells/ml. c.Calculate how many total cells you had in the original 250 µl of cell suspension. 1.Counting cells is an important part of many immunological assays, as is calculating dilution factors. a.You have 250 µl of a cell suspension. If you diluted 10 uls of that cell suspension into 90 µl of buffer, what would the dilution factor be? b.You then put 10 µl of that solution into the hemocytometer and you count 47 cells in the appropriate squares. Calculate the cells/ml. c.Calculate how many total cells you had in the original 250 µl of cell suspension.Explanation / Answer
a) dilution factor= volume of cell suspension added / total volume
= 10/ 100
= 1/10
b) No of cells in 1ml(1000Micro litre) = (47/10) x 1000
= 4700 cells/ ml
C)No. Of cells in 250 microliter= (47/100) x 250
= 1175 cells/ 250 microliter
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