Figure 2 shows the results of an experiment performed on a cell line that respon

Question

Figure 2 shows the results of an experiment performed on a cell line that responds to nerve growth factor (NGF) treatment resulting in differentiation and neurite growth: the cells turn into nerve cells The process of neuronal differentiation is accompanied by a number of biochemical changes. In this experiment the effect of NGF on the subsequent expression of the cell growth control gene, c -fos, was investigated. The role of a protein kinase, protein kinase C, in the process of gene expression control also was studied. Cells were grown in six tissue culture dishes. In samples 1-3, protein kinase C was active in the cells. In samples 4-6, protein kinase C was inactive in the cells. Samples 2 and 5 were treated with NGF for 30 minutes. Samples 3 and 6 were treated with phorbol ester (induces non-specific cell growth) for 30 minutes, Samples 1 and 4 were untreated controls. After incubation, cytoplasmic RNAs were prepared from the cells and subjected to Northern blot analysis using a radioactive c-fos gene fragment as a probe Treatment Protein kinase C + Statement: NGF causes an increase of c-fos gene transcription in these cells. A) The statement is supported by the information given. B) The statement is contradicted by the information givern C) The statement is neither supported nor contradicted by the information given.

Explanation / Answer

From the Northern blot analysis results it can be concluded that the expression levels of c-fos is increased in presence of nerve growth factor. So, the correct option is option A, the statement is supported by the information given.

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