electrode You are to design an amperometric sensor to measure oxygen from clinic
ID: 241731 • Letter: E
Question
electrode You are to design an amperometric sensor to measure oxygen from clinical blood specimens obtained during surgery a. Draw the sensor schematic and mark the components b. What information will your setup acquire? Choices are: hemoglobin bound oxygen, oxygen dissolved in blood outside red blood cells, or both. Write up the electrochemical reaction that gives rise to current and explain in one sentence in simple physical terms why the sensor's calibration will be linear c. d. Your device has the following calibration: 10uM corresponds to 7uA of current 3 uM corresponds to 4 HA of current and the output of your device has a standard deviation of 0.7 Calculate the oxygen concentration when 1. Current is 6.5 uA 2. Current is 0.5 Be careful when interpreting these measurements Physical design optimization: which is best from the following placements of the Pt microelectrode inside the sensor relative to the gas permeable membrane e. 0.5 mm I micron 0.5 cm 2 micron 0 micron (directly touching the membrane) These value are possible distances of the Pt microdisc from the gas membrane This is a question that requires just simple logicand no equations or prior study materialExplanation / Answer
b, A novel amperometric nitrite sensor was developed based on the immobilization of hemoglobin/colloidal gold nano particles on a glassy carbon electrode by a titania sol-gel film. The formation of morphology of the Hb-cs@fe3o4 composite was charecterized by scanning electro chemical microscopy,XRD and UV-VD spectroscopy.It is shown that Hb is fully integrated in to the cs@fe3o4 and can be manipulated by a magnetic field ehilst maintaining its biological activity.
c, The electrochemical sensoring technique has thye advantage if having a linear output and operating with low consumption. Consequently it operates on batteries for a long period of time.
e, 1micron
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