Answer the following questions using the information you have learned from the t

Question

Answer the following questions using the information you have learned from the transcription and translation lectures. The following double-strand sequence codes for a which is 13 amino acid long. To help you to choose the right combination of rt and Stop codons, all possible start and stop codons are marked as indicated below Start codon ATG, marked by underlines Stop codons- UAA.UAG UGA, marked by bold-types) You need to know which start codon with which stop codon will make a 13 amino acid long protein, and also you need to know which strand of DNA code for this protein. -CGGATGCACTGTAGATGCGCGccCCATAAAAAGTTTCACTGTTGATTA-3 3'-GCCTACGTGACATCTACGCGCGGGGTATTTITCAAAGTGACAACTAAT-5 5 Which strand codes for this protein? A) top B) bottom C) one of the above D) both strands 6. Which one of the following sequences is the right sequence for this mRNA? A) 5'-ATGCACTGTAGATGCGCGCCCCATAAAAAGTTTCACTGTTGA-3 B) 5'-ATGCGCGCCCCATAA-3 C) 5'-A E) none of th above 5"-CGGATGCACTGTAGATGCGCGCCCCATAAAAAGTTTCACTGTTGATTA-3 y.GCCTACGTGACATCTACGCGCGGGGTATTTTTCAAAGTGACAACTAAT. 5 , Which is the right anticodon for the 2nd amino acid for this protein during translation? A) 5-GTG-3 B) 5-GCG-3 C) 5-GUG-3 D) 5'-CAC-3 E) none of the above 8. One base insertion has occurred in the upper strand as shown in the following: ATGCACTGTAG to ATGCACTGTTAG What would be the outcome of this insertion? A)it will make a shorter-size protein B) it will not make any change C) it will make a missense mutation without changing size D) none of the above

Explanation / Answer

5) Option b - Bottom

Reason: The coding of protein is dependent on the sequence of mRNA. We know that in transcription antisense strand acts as a template for mRNA synthesis. This mRNA directs the synthesis of protein using a triplet codon. Start codon ATG is located in the antisense strand. Hence, option b is correct.

6) Option d

Reason: We have to use the bottom strand to find the sequence of mRNA. When we match the start codon and following sequencing of the DNA with mRNA, we will get the answer.

7) Option b - 5'-GCG-3'

Reason: When we check the sequence of mRNA (option d in question 6), the second triplet codon is CGC. The anticodon for this codon is GCG.

8) Option c - It will make a shorter-size protein

Reason: The insertion creates the stop codon UAG at the end. Hence, the formation of one amino acid will be stopped. So, the size of the protein reduces.

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